Plasmid vectors pET11a-link-NGFP and pMRBAD-link-CGFP and positive control vectors pET11a-Z-NGFP and pMRBAD-Z-CGFP are available upon request (Fig. 1) Figure 1: GFP-fragment reassembly vectors and ...

For lentivirus production, HEK293T cells were transfected with the CS–Hes1 or mock plasmid together with ... inducibly expressing Hes1, green fluorescent protein (GFP)-expressing THP1 cells ...