The oligonucleotide used to prime probe synthesis should be 20–25 nucleotides in length and complementary to the RNA strand to be analyzed. It should hybridize to the template DNA 250–500 ...

Specifically, one strand of a double-stranded siRNA molecule can be incorporated into a complex called RISC. This RNA-containing complex can then inhibit transcription of an mRNA molecule that has ...

RNA is similar to DNA but contains uracil instead of thymine (T) and has a single-stranded structure. Comparison of a single-stranded RNA and a double-stranded DNA with their corresponding nucleobases ...