He predicted that the restriction enzyme and the modification enzyme act on the same DNA sequence, called a recognition sequence. In this way, the bacterial cell's own self-defense mechanism ...

meaning that the sequence reads the same forward and backward. HindIII, for example, is an H. influenzae restriction enzyme that recognizes the sequence 5'AAGCTT-3' (upper strand)/3'TTCGAA-5 ...

The discovery of restriction enzymes in the early 1970s heralded a new age in molecular biology. These enzymes recognize characteristic DNA sequences and cut them. Bacteria and Archaea can also use ...

While Roberts pursued new restriction enzymes, others used them to sequence, map, clone, and manipulate genes. At the start of his tenure with Cold Spring Harbor Laboratory, Roberts originally ...

This process is referred to as cloning. It uses restriction enzymes to cut the plasmid and the gene fragment at sequence-specific locations that depend on the enzyme used. Then, enzymes called ligases ...