Total RNA from frozen tissues was extracted using TRIZOL reagent. RNA was extracted from formalin-fixed, paraffin-embedded tissues by digestion with proteinase K before the acid-phenol:chloroform ...

Global micro-RNA (miR) profiling of human malignancies is increasingly performed, but to date, the majority of such analyses have used frozen tissues. However, formalin fixation is the standard and ...

Immunoperoxidase and immunofluorescence techniques detected and localized infectious bursal disease virus (IBDV) in formalin-fixed paraffin-embedded sections of the bursa of Fabricius of ...

Scientists from Spain have recently compared the efficacy of the quantitative polymerase chain reaction (qPCR) method and immunohistochemistry in detecting severe acute respiratory syndrome ...

As technologies and methodologies advance, so do the potential applications for biospecimens. In this article, we explore seven uses of FFPE, ranging from the common to the cutting-edge.

Formalin-fixed paraffin-embedded (FFPE) tissues are the most widely used clinical sample types in histology and molecular diagnosis. However, due to RNA degradation and protein crosslinking, FFPE ...

The relatively new field of onco-metabolomics attempts to identify relationships between various cancer phenotypes and global metabolite content. Previous metabolomics studies utilized either nuclear ...

An invaluable source of RNA, DNA, and chromatin from historical and clinical samples is formalin fixed paraffin embedded (FFPE) tissue. Globally, tissue banks, hospitals, and laboratories are ...

Relationship between tumor gene expression and recurrence in stage II/III colon cancer: Quantitative RT-PCR assay of 757 genes in fixed paraffin-embedded (FPE) tissue ...

A single-arm trial of panitumumab in cetuximab refractory KRAS wild-type colorectal cancer. Background: Genomic profiling has improved our understanding of the underlying biology of tumors, accuracy ...