To measure enzyme activities, cell-free extracts were made from cellobiose-grown C. cellulolyticum strains at the mid-log phase using CelLytic B cell lysis reagent (Sigma). Crude extracts were ...

It is a unique member of the plastic family as it can be broken down into its constituent units (or monomers) by PETases, which are enzymes that degrade PET. Until now, scientists have discovered ...