Tandem copies of the insulator at each end of their ubiquitous CMV promoter- gfp transgene ensure uniform levels of green fluorescence in transgenic tadpoles, regardless of the chromosomal ...

For example, we initially examine protein localization in Drosophila KC cells using pHGW and pHWG (Hsp70 promoter, GFP fusions at either end). This way we can test which end of the protein better ...

(A) ADS was fused with GFP at the N-terminus with a linker GGSG in between. The fusion GFPADS and FppS (farnesyl pyrophosphate synthase) formed an operon under the IPTG-inducible promoter P hyperspank ...