To achieve polysome accumulation at ectopic sites in experiments, we overexpressed a useless protein (mTagBFP2) for the cell from a T7 promoter on a multi-copy pET28 plasmid (Figure 7A). The resulting ...

(A) ADS was fused with GFP at the N-terminus with a linker GGSG in between. The fusion GFPADS and FppS (farnesyl pyrophosphate synthase) formed an operon under the IPTG-inducible promoter P hyperspank ...

To silence BCAS2 expression, shRNA constructs in pLL 3.7-GFP plasmid were generated to target the following sequences: shRNA1, GAATGTGTAAACAATTCTA; shRNA2: GAAGGAACTTCAGAAGTTA. Transfection was ...

Yamaguchi M, Saito H, Suzuki M, and Mori K Visualization of neurogenesis in the central nervous system using nestin promoter-GFP transgenic mice Neuroreport 2000 11 1991-1996 ...

For example, we initially examine protein localization in Drosophila KC cells using pHGW and pHWG (Hsp70 promoter, GFP fusions at either end). This way we can test which end of the protein better ...