A method to select for proteins that are highly efficient at folding yields 'superfolder' GFP, an optimized GFP for use in protein fusions. Fusion proteins are powerful tools for the elucidation ...

These useful findings assigned a novel functional implication of histone acylation, crotonylation. Although the mechanistic insights have been provided in great detail regarding the role of the YEATS2 ...

This manuscript describes an important finding of the transcriptional control of a chimeric gene transfer agents (GTA) cluster in Bartonella by a processive anti-termination factor (BrrG). The ...

NOD1 expression plasmid and NF-κB or ISRE luciferase reporter plasmid were co-transfected into ... Hela cells were plated onto coverslips and incubated overnight, then NOD1-GFP expression plasmids and ...

10% of cell lysates were taken as input. Appropriate cell lysates were incubated for 24h with promoter DNA probe-bound beads, which were pre-made by mixing 30 μl M-280 streptavidin Dynabeads ...

Quantification was performed using the AIDA software (Raytest, V.5.0). N-terminally FLAG-tagged human NFIL3-T2A-GFP (WT or carrying the M170I mutation) was expressed transiently from a plasmid in ...

Plasmid DNA is an essential part of cell and gene therapy products. It is utilized as a template vector for cell therapy and non-viral gene therapy and as a raw material to produce adeno-associated ...

The Drosophila Gateway™ Vector collection is a set of 68 Gateway-based vectors designed to express epitope-tagged proteins in Drosophila culture cells or flies. At its core is Invitrogen's Gateway™ ...

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