Plasmid vectors pET11a-link-NGFP and pMRBAD-link-CGFP and positive control vectors pET11a-Z-NGFP and pMRBAD-Z-CGFP are available upon request (Fig. 1) Figure 1: GFP-fragment reassembly vectors and ...
Ad5-CMV-Empty Ad5-Empty virus contains a CMV promoter and poly(A) but no transgene ... microscope is a quick method for estimating transduction efficiency. Ad5F35-CMV-GFP Ad5F35-CMV-GFP virus is a ...
For lentivirus production, HEK293T cells were transfected with the CS–Hes1 or mock plasmid together with ... inducibly expressing Hes1, green fluorescent protein (GFP)-expressing THP1 cells ...
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