The discovery of restriction enzymes nearly forty years ago revolutionized the study of DNA and accelerated our ability to both sequence and synthesize genomes. These enzymes opened the path to a ...

While Roberts pursued new restriction enzymes, others used them to sequence, map, clone, and manipulate genes. At the start of his tenure with Cold Spring Harbor Laboratory, Roberts originally ...

Restriction enzymes. Various types of endonucleases – enzymes that can cut DNA – were already known before CRISPR-Cas9. The discovery of restriction enzymes in the early 1970s heralded a new age in ...

Restriction enzyme sequences can be added onto the ends of a genetic sequence through PCR, and they have been conveniently packed into vectors so genes can be easily inserted by using them. When using ...

Restriction enzymes have become important "scalpels" in genetic engineering, and have been commercialized and widely used in genetic engineering. Classification of restriction endonucleases.

Restriction enzymes are essential tools for recombinant DNA technology that have revolutionized modern biological research; however, they have limited sequence specificity and availability. The ...

During the past few years a novel approach based on the use of type IIS restriction enzymes in combination with T4 DNA ligase has become popular (known as Golden Gate cloning, PLoS ONE 3, e3647 ...

"We found a restriction enzyme that cuts the DNA a short distance from its recognition site, so we could design our sequences such that it would cut leaving no trace of additional DNA," McKinley said.

Today, scientists recognize three categories of restriction enzymes: type I, which recognize specific DNA sequences but make their cut at seemingly random sites that can be as far as 1,000 base ...