This valuable study reports on the critical role of ANKRD5 (ANKEF1) in sperm motility and male fertility. However, the supporting data remain incomplete. This work will be of interest to biomedical ...

The immunoprecipitated proteins were washed three times with Net Gel Buffer (150 mM NaCl, 50 mM Tris-HCl pH 7.5, 1mM EDTA, 0.1% NP40 and 0.25% gelatin) and once with RIPA Buffer (150 mM NaCl, 1% NP40, ...