Furthermore, a plasmid expressing only GFP (GFP-Control) was used as a control for the fluorescence pattern obtained by a protein with known cytoplasmic localization such as GFP (Kitamura et al., 2015 ...

The GFP plasmid was diluted 10 (0.1 μg) - and 5 (0.2 μg) - fold and transfected into 293T cells. As shown in Figure 2, there were few GFP-positive cells, and the 10-fold diluted GFP resulted in the ...

Mutagenizing sequences enriched with promoter motifs creates gene expression de novo, and helps to understand promoter logic, regulatory evolution, and the birth of de novo genes.

The first is the promoter iGem bba_j23110 on the top DNA strand, which drives moderate GFP expression from plasmid pMR1. The second is the reverse complement of this promoter, which drives moderate ...

Comparison of these results to Figure 1 D, where positive crosstalk is observed at low GFP plasmid concentrations, suggests that this significant negative crosstalk must primarily occur at the level ...

We mixed GFP and mCherry plasmids, with both genes under the control of the T7 promoter at varying plasmid ratios and a total plasmid concentration in the samples 1, 5, 10, or 15 nM (Figure 2 b).

CMV-GFP (control) and the p62 promoter-GFP plasmid (P p62 -GFP) were nucleotransfected into Id3 WT and Id3 KO murine B cells. We confirmed the ability of the constructs to produce eGFP protein using ...