2025年2月4日 · To mitigate the impact of the acetic acid metabolic pathway in E. coli, which adversely affects the cellular environment and depletes organic carbon, thereby reducing cytidine synthesis, this study employed a sequential knockout …

High concentrations of organic acids such as acetate inhibit growth of Escherichia coli and other bacteria. This phenomenon is of interest for understanding bacterial physiology but is also of practical relevance. Growth inhibition by organic acids underlies food preservation and causes problems during high-density fermentation in biotechnology.

2017年2月10日 · Our data indicate that acetate metabolism in E. coli is controlled to a large extent by the environment through the thermodynamic control of the Pta-AckA pathway. This control allows the...

2016年9月20日 · The metabolism of acetate in Escherichia coli has attracted the attention of the scientific community due to its role in central metabolism and its link to multiple physiological features. In this microorganism, acetate is involved directly or indirectly on the regulation of functional processes, such as motility, formation of biofilms, and ...

2025年1月25日 · The OAH yield of the engineered strain OAH28 was further increased to 17.49 g/L by strengthening the acetic acid reuse pathway, improving the supply of acetyl-CoA, and regulating the expression of MetX from different sources.

2022年12月1日 · For the first time, an engineered E. coli strain was used to construct a novel β-caryophyllene biosynthesis route based on the MVA system, which converts acetic acid to make β-caryophyllene [107].

1992年3月15日 · Escherichia coli selects acetic acid synthesis as the aerobic energy source because it generates the second largest amount of ATP and NADH(2). According to our proposition, acetic acid formation could be reduced by decreasing the anabolic requirement, i.e., reducing glucose uptake, or by increasing the capacity of the oxidative metabolism.

2023年6月12日 · Acetate, a major by‐product of glycolytic metabolism in Escherichia coli and many other microorganisms, has long been considered a toxic waste compound that inhibits microbial growth. This counterproductive auto‐inhibition represents a major problem in biotechnology and has puzzled the scientific community for decades.

2011年3月1日 · We performed growth selections upon an E. coli genome library developed using a moderate selection pressure to identify genomic regions implicated in acetate toxicity and tolerance.

Acetic acid can be metabolized to acetyl-CoA either by the PTA-ACKA pathway or by acetyl-CoA synthetase (ACS) through an intermediate acetyl-AMP.