2021年9月1日 · An exclusive native PCR modification known as Asymmetric PCR has been applied to amplify only a single strand during the PCR. PCR is a thermocycler, amplifies DNA. …

Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. [1] The technique has applications in some types of sequencing and …

2022年1月6日 · Asymmetric PCR is one of the most utilized strategies in ssDNA generation towards DNA aptamer generation due to its low cost, robustness and the low amount of …

Asymmetric PCR was used to preferentially amplify the sense strand of the original DNA to a greater extent than the anti-sense strand. Asymmetric PCR is useful in hybridisation probing in …

AELA-PCR represents a simple and efficient PCR methodology to generate single stranded amplicons for microarray applications. In contrast to standard asymmetric PCR methods, …

2024年5月21日 · In this study, a new multiplex asymmetric PCR approach that combines the amplification refractory mutation system (ARMS) with the homo-Tag-assisted nondimer …

2022年4月20日 · Asymmetric PCR is a variation of PCR used to preferentially amplify one strand of the original DNA more than the other. Asymmetric PCR differs from regular PCR by the …

2022年11月30日 · We report a novel variant of PCR called the Asymmetric Exponential and Linear Amplification (AELA) which can overcome these issues and generate large amounts of …

2004年2月9日 · Linear-After-The-Exponential (LATE)–PCR describes a new paradigm for primer design that renders assays as efficient as symmetric PCR assays, regardless of primer ratio. …

2019年1月24日 · Numerous research groups aimed at improving asymmetric PCR by identifying the appropriate primer ratios, template concentrations, PCR conditions, and number of …