3.将酚红和溴分别溶于醋酸中，制成溶液。先将酚红醋酸溶液加热至沸，然后在搅拌下缓慢加入溴的醋酸溶液：当酚红反应完全且黄色晶体不再析出后，冷却，过滤。

Bromophenol blue (3′,3″,5′,5″-tetrabromophenolsulfonphthalein, BPB), [2] albutest [3] is used as a pH indicator, an electrophoretic color marker, and a dye. It can be prepared by slowly adding excess bromine to a hot solution of phenolsulfonphthalein in glacial acetic acid.

Bromophenol Blue | C19H10Br4O5S | CID 8272 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.

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Bromophenol Blue (BPB): sulfonated hydroxyquinone, acts as a pH indicator and tracking dye in gel electrophoresis. Widely used in DNA, RNA, and protein analysis.

2025年1月20日 · Bromophenol Blue is a versatile compound widely used in scientific research as a pH indicator. Known for its ability to change color depending on the pH of the solution, it plays a critical role in various laboratory applications, including electrophoresis, titration, and …

Bromophenol blue is an intermediate and is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio.

溴酚蓝（Bromophenol blue），生物学中常用的一种示踪染料、酸碱指示剂。 作为示踪染料，本品可作为一种颜色标记用于核酸（琼脂糖）、蛋白质（聚丙烯酰胺）凝胶电泳；作为一种pH指示剂，其有效指示范围在3.0-4.6，其颜色变化从黄色（pH3.0）到蓝紫色（pH4.6）。

Bromophenol blue, Tetrabromophenolsulfonephthalein, is an acid-base indicator whose useful range as an indicator lies between pH 3.0 and 4.6. It changes from yellow at pH 3.0 to purple at pH 4.6; this reaction is reversible.

Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.