Lectins: Concanavalin A (Con A), Biotinylated - VectorLabs
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN …
[Lectin blotting]:Glycoscience Protocol Online Database - JCGGDB
Lectins are carbohydrate binding proteins, and can be used to discriminate and analyze the glycan structures of glycoproteins. The lectin blotting technique detects glycoproteins …
A Useful Guide to Lectin Binding: Machine-Learning Directed …
2022年1月27日 · Isolated in 1936, ConA is perhaps the most commonly used lectin. ConA is often annotated as a high-mannose binding lectin, although its true specificity is more complex. …
Lectins: Concanavalin A (Con A), Fluorescein Labeled - VectorLabs
Concanavalin A (Con A), Fluorescein (FL-1001-25) Con A recognizes α-linked mannose present as part of a “core oligosaccharide” in many serum and membrane glycoproteins. Fluorescein …
Lectin affinity chromatography and quantitative proteomic analysis ...
2020年10月5日 · Concanavalin A (Con A) lectins can capture peptides with high-mannose type N-glycan, while wheat germ agglutinin (WGA) can capture N-Acetyl glucosamine (GlcNAc) and …
ConA-Like Lectins: High Similarity Proteins as Models to Study
2018年12月21日 · Diocleinae lectins, also called ConA-like lectins, present a high similarity of sequence and three-dimensional structure and are known to present inflammatory, vasoactive, …
Combined biochemical and cytological analysis of membrane …
2013年10月1日 · Lectin blotting with ConA showed that a subset of Triton X-114-soluble membrane proteins bound the lectin, demonstrating the presence of Triton X-114-soluble high …
Lectin blotting - Glycoscience Protocols (GlycoPODv2) - NCBI Bookshelf
2021年9月10日 · In this chapter, we describe a lectin blotting method using biotinylated lectin followed by detection with horseradish peroxidase (HRP)-labeled Streptavidin. Here, signal …
华盈视角：糖蛋白标志物研究工具——凝集素芯片 - 知乎
2019年8月8日 · 糖基化是较广泛和复杂的蛋白质共翻译或翻译后修饰之一，它是通过糖基转移酶，在新生多肽或者成熟蛋白质的特定氨基酸上添加多个单糖的过程，通俗的理解就是在细胞器 …
科普多种凝集素-荧光标记技术及标记凝集素介绍 - 搜狐
2021年1月29日 · 凝集素lectin是一种无免疫原性蛋白质，分子量为11 000~335 000之间，可从植物或动物中提取，具有凝集红细胞的特性。 故又称植物血凝素。 凝集素能特异地与糖蛋白中的 …