A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria ; however, plasmids are sometimes present in archaea and eukaryotic organisms .

6 天之前 · A plasmid is a small circular DNA molecule found in bacteria and some other microscopic organisms. Plasmids are physically separate from chromosomal DNA and replicate independently. They typically have a small number of genes — notably, some associated with antibiotic resistance — and can be passed from one cell to another.

2022年12月21日 · We have developed an efficient fully synthetic method and protocol that enables the production of circularized DNA containing expression elements ready for transfection in as little as 3 hours,...

We have developed an efficient fully synthetic method and protocol that enables the production of circularized DNA containing expression elements ready for transfection in as little as 3 hours, thereby eliminating the bacterial cloning steps.

In this review, we focus on replication and its control in circular plasmids. Plasmid replication can be conveniently divided into three stages: initiation, elongation, and termination. The inability of DNA polymerases to initiate de novo replication makes necessary the independent generation of a …

2025年1月2日 · A dimeric Wadjet complex, which is a minimal functional unit for loop extrusion, reels in DNA simultaneously from both sides on both circular and linear DNAs, whereas DNA cleavage occurs only for the plasmid DNA, specifically at the anchor point of the plasmid DNA where loop extrusion was stalled.

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes.

2021年6月23日 · Not all plasmids are circular but all plasmids contain extra-chromosomal DNA. Chloroplast DNA is contained within a chromosome and is not a plasmid.

2021年5月25日 · The same reaction is used to re-form a circular plasmid after conjugational transfer. The displaced circular single-stranded DNA now replicates by a completely different mechanism using only host-encoded proteins.

Plasmids are small, circular arrangements of DNA that are not integrated into an organism’s genome and can replicate independently of genomic DNA. Plasmid DNA is mainly found in bacteria, carrying genes that serve survival functions, …