Visualizing neuroinflammation with fluorescence and luminescent ...
Mouse BV2 microglia phenotype cells and mouse J774 macrophage cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) with high glucose (4500 mg/l), … 展开
Primers for ISH were designed for this study using Primer3 (NCBI Nucleotide), spanning exon-exon junctions and including the protein-coding regions of the mouse … 展开
Cells were first washed in PBS pH 7.2 and fixed using 4% formaldehyde for 10 min and then again washed with PBS. They were permeabilized with 70% EtOH for 30 … 展开
Pathogen-free adult male BALB/c mice (18–25 g, aged 8–10 weeks) were utilized for the experiments in this study. Mice were housed under SPF conditions in … 展开
On day 21 of testing, the mice were perfused and then incubated with 4% paraformaldehyde (PFA) for standard fixation; n = 3 animals from each group were selected for … 展开
Multichannel silk protein/laminin grafts for spinal cord injury repair
The physical, chemical, and bioactive cues provided by biomaterials are critical for spinal cord regeneration following injury. In this study, we investigated the bioactivity of a silk-based …
- 作者: Qiang Zhang, Qiang Zhang, Shuqin Yan, Renchuan You, David L. Kaplan, Yu Liu, Jing Qu, Xiufang Li, Mi...
- Publish Year: 2016
Staining of glial fibrillary acidic protein (GFAP) in lumbar spinal ...
1991年11月22日 · This injury model of peripheral neuropathy resulted in a behavioral hyperalgesia evidenced by a decrease in mean foot withdrawal latency to radiant heat. …
- Laminin and GFAP Staining Spinal Cord Inury Lesion Volume 的研究
Staining of glial fibrillary acidic protein (GFAP) in lumbar spinal ...
1991年11月22日 · Increased GFAP immunostaining was observed in the gray matter of the spinal cord ipsilateral to the lesion and specific to spinal segments in which the sciatic nerve is …
- 作者: Chris Garrison, P. M. Dougherty, K. C. Kajander, S. M. Carlton
- Publish Year: 1991
TGF-α increases astrocyte invasion and promotes axonal growth …
The axon-rich matrix was associated with increased laminin immunoreactivity throughout the lesion site. Thus, administration of exogenous TGF-α alters the evolution of the local …
- 作者: Robin E. White, Feng Qin Yin, Lyn B. Jakeman
- DOI: 10.1016/j.expneurol.2008.06.012
- Publish Year: 2008
- 已发布: 2008/11
Laminin-coated multifilament entubulation, combined with …
2020年8月10日 · These results demonstrate that the combinatorial strategy of filament-filled guiding scaffold, adhesive molecular laminin, Schwann cells, and glial cell line-derived …
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Transforming Growth Factor α Transforms Astrocytes to a Growth ...
2011年10月10日 · This approach allows visualization of the rostrocaudal distribution, which reflects an estimate of the volume fraction (Vv) of NF and laminin staining as a function of the …
Astrocyte-derived lipocalin 2 promotes inflammation and scarring …
2024年10月1日 · An AAV carrying the GFAP promoter was constructed and administered to the spinal cord of mice two weeks prior to inducing the model. This intervention resulted in a …
Multichannel silk protein/laminin grafts for spinal cord injury repair ...
2016年7月30日 · The physical, chemical, and bioactive cues provided by biomaterials are critical for spinal cord regeneration following injury. In this study, we investigated the bioactivity of a …
Polylaminin, a polymeric form of laminin, promotes …
2010年7月19日 · Fluorogold-labeled neurons were counted in a Zeiss Axiovert 200, equipped with LSM 510 Meta NLO system for confocal and multiphoton imaging (Carl Zeiss, Oberkochen, Germany). Three regions were selected for …
