A lateral flow test (LFT), [1] is an assay also known as a lateral flow immunochromatographic test (ICT), or rapid test. It is a simple device intended to detect the presence of a target substance in a liquid sample without the need for specialized and costly equipment.

The lateral flow assay (LFA) is a paper-based platform for the detection and quantification of analytes in complex mixtures, where the sample is placed on a test device and the results are displayed within 5–30 min. Low development costs and ease of production of LFAs have resulted in the expansion of its applications to multiple fields in ...

2016年9月1日 · A lateral flow assay is composed of a chromatographic system (separation of components of a mixture on the basis of differences in their movement through reaction membrane) and immunochemical reaction (between antibody-antigen, nucleic acid-target analyte) [4], [5], [6]. It is based on the movement of sample across the membrane via capillary force.

2024年5月2日 · A lateral flow test (LFT) is a simple, quick and cheap assay that can detect the presence of target antigen(s) or antibody(ies) in a liquid sample. 1 As a type of immunoassay, detection relies on the binding of antibodies with their target antigen combined with a detection reaction to produce a readable, often visible, result.

Lateral flow immunoassays used in clinical microbiology laboratories are usually double-antibody sandwich assays. For the detection of antigens, the capture zone (test line) on the membrane contains immobilized antibodies.

Lateral flow assays (LFA) are the most recently used approaches for biosensing and quantifying numerous analytes, including different viruses . The technical principle of LFAs was derived from the latex agglutination test.

2016年6月30日 · Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices popular in biomedicine, agriculture, food and environmental sciences. This review presents an overview of the principle of the method and the critical components of the assay, focusing on lateral flow immunoassays.